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KMID : 0358920160430040419
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Journal of the Korean Academy of Pedodontics
2016 Volume.43 No. 4 p.419 ~ p.426
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Relationship with Passage Time of Human Dental Pulp Stem Cells from Supernumerary Tooth by Classification
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Shin Yeo-Seob
Kim Jong-Bin
Kim Jong-Soo
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Abstract
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For this research 20 supernumerary teeth impacted in the maxillary anterior have been extracted and pulp cells have been collected from them. From the collected pulp cells, total of 17 (10 males, 7 females) have been selected as subjects. From this research, the run-time of successive culture of the cell from tooth number pulp tissue was 2.91 ± 0.29 days. From the gathering of cells from the initial pulp tissue until gaining 80% confluency took 4.53 ± 0.94 which was the longest. The following successive cultures took 2.73 ± 0.32 days. Average runtime for female was 2.81 ± 0.27 days whereas male had average runtime of 2.98 ± 0.29 days. Average run-time for inversion was 2.94 ± 0.30 days and for normal location, 2.80 ± 0.20 days. Average runtime was 2.92 ± 0.31 days and other forms took 2.88 ± 0.22 days. In the future, follow up research would be needed to evaluate the efficiency of the cells collected from the initial passage and the latter passage as stem-cells and taking into consideration the less than 3 days’time for the subculture, it could be concluded that the research efficiency and fast cultivation would be sufficiently effective.
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KEYWORD
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Supernumerary tooth , Passages , Classification , Stem cells
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